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KMID : 1094719960010010041
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Biotechnology and Bioprocess Engineering
1996 Volume.1 No. 1 p.41 ~ p.45
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Effects of medium components on L-ornithine production byBrevibacterium ketoglutamicum
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Lee Tae-Ho
Chang Yong-Keun
Ryu Wook-Sang
Chung Bong-Hyun
Park Young-Hoon
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Abstract
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Effects of yeast extract, and ammonium sulfate were investigated on the production of L-ornithine by an arginine auxotroph.Brevibacterium ketoglutamicum in flask and batch cultures. Yeast extract as an arginine source and ammonium sulfate as an inorganic nitrogen source had significant effects on L-ornithine, production and cell growth. L-ornithine production was repressed by the excessive addition of arginine. Reversion of auxotrophic cells to the wild type was observed when the initial yeast extract concentration was too low. There existed optimum concentrations of yeast extract and ammonium sulfate for L-ornithine production. The effects of yeast extract and ammonium sulfate concentrations on the Leudeking-Piret model parameters were examined to analyze, the relationship between cell growth and L-ornithine production.
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KEYWORD
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L-ornithine, Brevibacterium ketoglutamicum, auxotroph, L-arginine
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